Spectrophotometry is the study of reflection or transmission properties of the substance as a function of wavelength. Spectrophotometry is an approach to measure how much a chemical substance absorbs light by means of measuring the power of light as a beam of light passes through test solution. Spectrophotometry uses photometers that can measure a gentle beam’s intensity being a function of its color generally known as spectrophotometers. Important top features of spectrophotometers are spectral bandwidth, along with the percentage of sample-transmission, along with the logarithmic range regarding sample-absorption and sometimes a portion of reflectance measurement.
Working Rule of Spectrophotometry
