Basic purpose of this lecture is to present on Reverse Transcription. A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription. It is mainly associated with retroviruses. Normal transcription involves synthesis of RNA from DNA. Reverse transcription is the transcription of single stranded RNA into double stranded DNA. With the help of the enzyme Reverse Transcription.