Principle objective of this lecture is to presentation on Biomedical Imaging. Other objectives are explain Images by various imaging modalities, Fundamental concepts on imaging and Image analysis by MATLAB: analysis, noise removal and feature extraction. This lecture briefly explain on Computer Aided Diagnosis (CAD), Signal Wavelength , Magnetic Resonance Imaging (MRI), Ultrasound Imaging, Light Microscopy, X-Ray Imaging and Nuclear Medicine Imaging.